ABSTRACT
The first documented cases of Coronavirus disease of 2019 (COVID-19) in Wuhan city (Hubei province, China) were caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), studies mention viral transmission is more common in indoor and poorly ventilated environments compared to outdoor environments or environments with abundant airflow. In this context, this research aimed to estimate, through a mathematical model by a Box-Behnken design, the time and occupancy required in a physical space to reach CO2 levels that exceed the risk level established as a safer condition of 700 ppm for SARS-CoV-2 transmission. According to the proposed mathematical model, it is possible to predict safe conditions. With this, it was found that natural ventilation is the best option to reduce CO2 concentration, considering the occupancy/m3 and time, allowing a constant airflow;the use of air conditioners to control the temperature in rooms without natural ventilation is suggested;notwithstanding, these types of equipment are not designed to reduce CO2 concentration. Thus, their use in rooms with open windows and doors leads to a shorter equipment lifetime, for this reason, its operation in special conditions, such as in rooms without natural ventilation should be considered
ABSTRACT
The COVID-19 pandemic has caused a decrease in tourism activity. This has caused huge losses to the tourism industry, so different approaches have been devised for economic reactivation. This article aims to provide a novel strategy for mass molecular monitoring of clinically healthy individuals, and also including potentially asymptomatic carriers of SARS-CoV-2, who traveled to a tourist resort The monitoring consisted of collecting individual saliva samples (n=120) and conforming groups of 10 samples, thus setting 12 individual pools, which were analyzed by qRT-PCR. Obtained data showed that all the samples analyzed were negative, thus confirming that no individuals were infected with SARS-CoV-2 before and during the travel. The proposed molecular protocol allowed the analysis of massive saliva samples and detect individual infected persons (negative or positive). protocol for evaluate massive event, thus promoting the economic activation of the tourism industry.